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Published on: February 20, 2012
Identification and characterization of microsatellite loci in the rush Juncus effusus (Juncaceae)
Stefan G Michalski1, Walter Durka
1Department of Community Ecology (BZF), Helmholtz Centre for Environmental Research UFZ, Theodor-Lieser-Strasse 4, D-06120 Halle, Germany. stefan.michalski@ufz.de
Premise Of The Study:
Microsatellite markers were developed for the common wetland plant Juncus effusus to assess levels of within- and among-population genetic diversity.
Methods And Results:
Using a next-generation sequencing approach we identified new microsatellite loci, from which 23 were further characterized in a large population of J. effusus. Expected heterozygosity ranged from 0 to 0.64 with a mean of 0.407. Inbreeding coefficients (mean = 0.984) were very high, confirming earlier suggestions of an inbred mating system. Cross-amplification was tested in J. conglomeratus, J. inflexus, J. drummondii, and J. filiformis, with best results in the former.
Conclusions:
The described microsatellite markers will be helpful in assisting studies on, e.g., reproduction, taxonomy, and phylogeography in J. effusus and related species.
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