Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Paris Saponins enhance radiosensitivity in a gefitinib-resistant lung adenocarcinoma cell line by inducing apoptosis and G2/M cell cycle phase arrest.

Molecular medicine reports·2016
Same author

Quantitative Proteomic Analysis Reveals Populus cathayana Females Are More Sensitive and Respond More Sophisticatedly to Iron Deficiency than Males.

Journal of proteome research·2016
Same author

Compact plane illumination plugin device to enable light sheet fluorescence imaging of multi-cellular organisms on an inverted wide-field microscope.

Biomedical optics express·2016
Same author

Gaussian-Charge Polarizable and Nonpolarizable Models for CO2.

The journal of physical chemistry. B·2016
Same author

Incorporating Human Movement Behavior into the Analysis of Spatially Distributed Infrastructure.

PloS one·2016
Same author

Evaluating the effect of clinical care pathways on quality of cancer care: analysis of breast, colon and rectal cancer pathways.

Journal of cancer research and clinical oncology·2016

Related Experiment Video

Updated: May 25, 2026

High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings
09:01

High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings

Published on: April 16, 2017

A new twist on scanning thermal microscopy.

Michael E McConney1, Dhaval D Kulkarni, Hao Jiang

  • 1Materials and Manufacturing Directorate, Air Force Research Laboratory, WPAFB, Ohio 45433, USA. michael.mcconney@wpafb.af.mil

Nano Letters
|January 27, 2012
PubMed
Summary

Researchers developed novel twisting thermal bimorphs to overcome limitations in scanning thermal microscopy. This innovation separates thermal and topographical signals, improving imaging accuracy for thermal mapping applications.

More Related Videos

Scanning Transmission Electron Microscopy Tomography in Virology: 3D Imaging of High-pressure Frozen, Freeze-substituted Samples
09:17

Scanning Transmission Electron Microscopy Tomography in Virology: 3D Imaging of High-pressure Frozen, Freeze-substituted Samples

Published on: August 6, 2025

Subsurface Defect Localization by Structured Heating Using Laser Projected Photothermal Thermography
11:34

Subsurface Defect Localization by Structured Heating Using Laser Projected Photothermal Thermography

Published on: May 15, 2017

Related Experiment Videos

Last Updated: May 25, 2026

High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings
09:01

High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings

Published on: April 16, 2017

Scanning Transmission Electron Microscopy Tomography in Virology: 3D Imaging of High-pressure Frozen, Freeze-substituted Samples
09:17

Scanning Transmission Electron Microscopy Tomography in Virology: 3D Imaging of High-pressure Frozen, Freeze-substituted Samples

Published on: August 6, 2025

Subsurface Defect Localization by Structured Heating Using Laser Projected Photothermal Thermography
11:34

Subsurface Defect Localization by Structured Heating Using Laser Projected Photothermal Thermography

Published on: May 15, 2017

Area of Science:

  • Materials Science
  • Nanotechnology
  • Physics

Background:

  • Thermal bimorphs are widely used for thermal sensing in devices like thermometers and infrared cameras.
  • Conventional scanning thermal microscopy (SThM) utilizes bending bimorphs, but this bending interferes with topographical data acquisition.
  • This interference limits the precision and applicability of current SThM techniques.

Purpose of the Study:

  • To address the signal interference issue in scanning thermal microscopy.
  • To develop a new type of thermal bimorph that overcomes the limitations of bending-based designs.
  • To demonstrate the advantages of a novel twisting bimorph approach for SThM.

Main Methods:

  • Fabrication of novel bimorph structures designed to twist rather than bend in response to thermal stimuli.
  • Implementation of these twisting bimorphs in a scanning thermal microscopy setup.
  • Comparative analysis of imaging performance between twisting and conventional bending bimorphs.

Main Results:

  • The developed bimorphs exhibit twisting motion in response to temperature changes.
  • This twisting mechanism effectively decouples thermal sensing from topographical imaging.
  • The twisting bimorphs demonstrate superior performance compared to traditional bending bimorphs in SThM.

Conclusions:

  • Twisting thermal bimorphs offer a significant advancement for scanning thermal microscopy.
  • This new approach enhances the accuracy of thermal and topographical measurements.
  • The technology holds promise for improved thermal imaging and material characterization.