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Related Concept Videos

Meiosis II01:57

Meiosis II

Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 chromosomes—one from each homologous initial pair. Importantly, each chromosome in these cells is composed of two joined copies, and when these cells enter meiosis II, the goal is to separate such sister chromatids using the same microtubule-based network employed in other division processes. The result of meiosis II is two haploid cells, each containing...

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Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling
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Rapid freezing without cooling equilibration in canine sperm.

Suhee Kim1, Yongcheol Lee, Honghyun Yang

  • 1Department of Veterinary Obstetrics and Theriogenology, College of Veterinary Medicine, Chonbuk National University, Jeonju, Jeonbuk, Republic of Korea.

Animal Reproduction Science
|January 27, 2012
PubMed
Summary

A new rapid canine sperm freezing method using 5% glycerol and liquid nitrogen vapor improves motility and viability. Holding sperm in liquid nitrogen vapor for 2 minutes is sufficient for successful rapid freezing without cooling equilibration.

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Area of Science:

  • Veterinary Science
  • Reproductive Biology
  • Cryobiology

Background:

  • Effective cryopreservation of canine semen is crucial for artificial insemination and genetic preservation.
  • Traditional methods often require slow cooling equilibration, which can be time-consuming and technically demanding.

Purpose of the Study:

  • To develop a simplified, rapid canine semen freezing protocol without cooling equilibration.
  • To evaluate the efficacy of different cryoprotective agents (CPAs) and freezing times in liquid nitrogen (LN2) vapor.

Main Methods:

  • Canine ejaculates were treated with CPAs (glycerol, ethylene glycol) or no CPA.
  • Semen was frozen using modified vitrification, involving exposure to LN2 vapor for varying durations before plunging into LN2.
  • Post-thaw sperm quality was assessed for motility, viability, morphology, and membrane integrity.

Main Results:

  • 5% glycerol significantly improved sperm motility, plasma membrane, and acrosome membrane integrity.
  • Freezing in LN2 vapor enhanced sperm motility, viability, and plasma membrane integrity compared to direct plunging.
  • Holding for 2 minutes in LN2 vapor was optimal for sperm quality, while longer durations increased abnormalities.

Conclusions:

  • A rapid canine sperm freezing method utilizing 5% glycerol and 2-minute LN2 vapor exposure is effective and simple.
  • This technique eliminates the need for cooling equilibration, offering a practical alternative for canine semen cryopreservation.
  • The findings provide a basis for further research into vitrification of larger volumes of canine sperm.