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Updated: May 25, 2026
A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum
Published on: April 10, 2018
Recombinant dengue type 2 viruses with altered e protein domain III epitopes are efficiently neutralized by human
Wahala M P B Wahala1, Claire Huang, Siritorn Butrapet
1Address correspondence to Aravinda M. de Silva, desilva@med.unc.edu.
Abstract:
Humans develop polyclonal, serotype-specific neutralizing antibody responses after dengue virus (DENV) infection. Many mouse antibodies that neutralize DENV bind to the lateral ridge or A strand epitopes on domain III of the viral envelope (EDIII) protein. It has been assumed that these epitopes are also the main target of human neutralizing antibodies. Using recombinant dengue serotype 2 viruses with altered EDIII epitopes, we demonstrate that EDIII epitopes are not the main target of human neutralizing antibody.
