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Updated: May 25, 2026

In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors

In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors

Published on: June 17, 2019

Reprogramming axonal behavior by axon-specific viral transduction.

B A Walker¹, U Hengst, H J Kim

¹Department of Pharmacology, Weill Medical College, Cornell University, New York, NY 10065, USA.

|January 27, 2012

Summary

This summary is machine-generated.

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Researchers developed a novel gene therapy targeting protein expression directly in axons, bypassing the cell body. This method uses modified viruses to enable local protein synthesis in axons, offering new therapeutic strategies for axonal disorders.

Area of Science:

Neuroscience
Molecular Biology
Gene Therapy

Background:

Current treatments for axonal disorders are limited by the inability to target therapeutic protein expression directly to injured axons.
Existing gene therapy methods rely on viral gene infection and transcription within the neuron's cell body, not the axon itself.

Purpose of the Study:

To develop and validate a method for targeting gene expression selectively to axons.
To explore the potential of local axonal protein synthesis for therapeutic interventions in axonal disorders.

Main Methods:

Utilized genetically engineered mice with epitope-labeled ribosomes to confirm the presence of ribosomes in distal axons.
Employed a modified Sindbis virus with an RNA genome capable of direct translation for local protein synthesis.

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Last Updated: May 25, 2026

In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors

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Applied the virus selectively to axons in both cultured neurons and living animals.

Main Results:

Demonstrated that the modified Sindbis virus enables local translation of heterologous proteins in axons.
Showed that selective axonal protein expression can modify axonal signaling, promoting axonal growth over inhibitory substrates.
Confirmed successful heterologous protein expression in the axons of living animals.

Conclusions:

Identified a novel strategy for manipulating protein expression directly within axons.
Established a new gene therapy approach for treating disorders affecting axonal function and integrity.
This method offers a promising avenue for in vivo alteration of the axonal proteome.