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Published on: September 27, 2019
Rectangular coordination polymer nanoplates: large-scale, rapid synthesis and their application as a fluorescent
Yingwei Zhang1, Yonglan Luo, Jingqi Tian
1State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, Jilin, People's Republic of China.
Plos One
|January 27, 2012
Summary
Researchers developed a novel fluorescent sensor using rectangular coordination polymer nanoplates (RCPNs) for highly sensitive and selective DNA detection. This platform achieves a low detection limit and distinguishes single-base mismatches in biological samples.
Area of Science:
- Materials Science
- Nanotechnology
- Analytical Chemistry
Background:
- Coordination polymers offer tunable properties for advanced applications.
- Developing sensitive and selective biosensors is crucial for early disease detection.
- Fluorescence-based sensing provides a sensitive method for biomolecule detection.
Purpose of the Study:
- To report the first large-scale, rapid synthesis of uniform rectangular coordination polymer nanoplates (RCPNs).
- To demonstrate RCPNs as an effective fluorescent sensing platform for multiple DNA detection.
- To investigate the mechanism of fluorescence quenching and validate the sensor in biological samples.
Main Methods:
- Synthesis of RCPNs from Cu(II) and 4,4'-bipyridine.
- Utilizing fluorescence quenching and recovery for DNA detection.
- Experimental elucidation of the fluorescence quenching mechanism.
- Testing the sensor's performance in human blood serum.
Main Results:
- Uniform rectangular coordination polymer nanoplates (RCPNs) were synthesized rapidly and at large scale.
- The RCPN platform achieved a low DNA detection limit of 30 pM.
- High selectivity was demonstrated, capable of detecting single-base mismatches.
- Successful application in a human blood serum matrix was shown.
Conclusions:
- The synthesized RCPNs are effective for sensitive and selective fluorescent DNA detection.
- The sensing mechanism relies on fluorescence quenching and recovery upon target hybridization.
- This platform shows promise for real-world diagnostic applications, including in serum.
