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[Determination of serum vitamin B1 and B2 with HPLC]
Liyan Liu1, Changhao Sun, Cheng Wang
1School of Public Health, Harbin Medical University, Harbin 150081, China. yanziliu2100@163.com
Objective:
To develop a HPLC method for the determination of serum vitamin B1 (VB1) and vitamin B2 (VB2) simultaneously.
Methods:
Serum samples were pretreated at ambient temperature on a C18 reversed phase chromatographic column, methanol plus phosphate buffer was used for mobile phase, vitamin B1 and B2 contents of serum samples eluted from a Sep-park C18 column were detected with a photodiode array detector. The influences of retention time, the volume and the proportion of eluent on the recovery of samples were investigated.
Results:
Recoveries were ranged from 82.67 to 96.89%. The coefficient of variation was < 10%. The linearity and correlation coefficient was 0.9985 and 0.9995, respectively. The contents of serum VB1 and VB2 in obese people were lower than those in healthy controls.
Conclusion:
A HPLC method of determining serum VB1 and VB2 was established. Serum VB1 and VB2 level in obese people was low.