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Updated: May 25, 2026

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
Published on: March 9, 2017
[Kinetic chemiluminescence as a method for the study of free radical reactions]
Abstract:
The review gives concepts of analysis of kinetics of complex reactions with the participation of free-radicals. The concepts are based on the comparison of the kinetic curves of the chemiluminescent reactions in the presence of a physical enhancer coumarin C-525 with the simulated kinetic functions. This method was applied to the investigation of the mechanism of a branched-chain reaction of lipid peroxidation in biological and phospholipid membranes as well as to the effect of antioxidants and determination of their activities. It was also used for studying the reaction of the formation of free radicals in the complex of cytochrome c with cardiolipin. This reaction plays a key role in the initiation of apoptosis.
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