Enzyme linked immunosorbent assay for immunoglobulins G to Mycoplasma hominis

Sera (187) from women patients attending the STD, Obstetrics and Gynaecology, and Family Planning Clinics were screened for the presence of the IgG class of immunoglobulins against M. hominis, using the ELISA technique. Sonicates of locally isolated M. hominis serotype (CS1) and standard PG 21 strain were used as antigens. The test was standardized using penicillinase as an enzyme. The ELISA showed 90.48 per cent sensitivity and 84.8 per cent specificity, and was also rapid (as compared to culture) and reproducible.