Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prevalence of anterior uveitis in dogs diagnosed with pyometra.

The Journal of small animal practice·2025
Same author

Molecular Dynamics of a Polymer Blend Model on a Solid Substrate.

Molecules (Basel, Switzerland)·2025
Same author

Current knowledge and practice of Candida auris screening in France: A nationwide survey from the French Society of Medical Mycology (SFMM).

Journal de mycologie medicale·2024
Same author

Role of IL33 in chronic inflammation and microvascular damage as a reflection of organ damage on a cohort of patients with acromegaly.

Journal of endocrinological investigation·2024
Same author

Impact of the physical properties of contact lens materials on the discomfort: role of the coefficient of friction.

Colloids and surfaces. B, Biointerfaces·2023
Same author

Erectile function and androgen and estrogen beta receptor gene polymorphisms in acromegalic men.

Journal of endocrinological investigation·2023

Related Experiment Video

Updated: May 25, 2026

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water
08:48

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water

Published on: April 28, 2022

Effective interactions in lysozyme aqueous solutions: a small-angle neutron scattering and computer simulation study.

M C Abramo1, C Caccamo, D Costa

  • 1Dipartimento di Fisica, Università degli Studi di Messina and CNISM (Consorzio Nazionale Interuniversitario di Struttura della Materia) Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy. mcabramo@unime.it

The Journal of Chemical Physics
|January 28, 2012
PubMed
Summary
This summary is machine-generated.

We observed changes in protein interactions in lysozyme solutions using neutron scattering. Simulations revealed how repulsive and attractive forces shift, influencing protein behavior and density fluctuations.

More Related Videos

Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2
11:27

Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2

Published on: December 8, 2016

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling
10:27

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling

Published on: October 21, 2018

Related Experiment Videos

Last Updated: May 25, 2026

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water
08:48

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water

Published on: April 28, 2022

Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2
11:27

Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2

Published on: December 8, 2016

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling
10:27

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling

Published on: October 21, 2018

Area of Science:

  • Biophysics
  • Soft Matter Physics
  • Solution Chemistry

Background:

  • Understanding protein-protein interactions is crucial for various biological and industrial processes.
  • Lysozyme solutions exhibit complex phase behavior influenced by pH and ionic strength.
  • Previous studies have explored lysozyme interactions, but a unified microscopic explanation remains elusive.

Purpose of the Study:

  • To investigate the protein-protein structure factors in aqueous lysozyme solutions under varying conditions.
  • To elucidate the microscopic origins of observed changes in lysozyme interactions and solution behavior.
  • To compare the predictive power of different coarse-grained models for lysozyme solutions.

Main Methods:

  • Small-angle neutron scattering (SANS) experiments were conducted on aqueous lysozyme solutions.
  • Extensive simulations using coarse-grained models were performed to rationalize experimental findings.
  • Three distinct models were explored: soft spheres with attractive potential and charges, Derjaguin-Landau-Verwey-Overbeek (DLVO) model, and a mixed model.

Main Results:

  • SANS experiments revealed an upturn in structure factor at low wavevectors with increasing pH, indicating a shift towards attractive interactions and enhanced density fluctuations.
  • The soft sphere model qualitatively predicted the observed structure factor upturn.
  • The DLVO model accurately predicted the protein-rich/protein-poor coexistence curve but struggled with experimental correlations at low pH/ionic strength.
  • The mixed model, incorporating DLVO attraction into a distributed-charge approach, showed improved agreement with the experimental crossover.

Conclusions:

  • The study highlights a crossover in lysozyme solution behavior from repulsive to attractive interaction dominance with increasing pH.
  • Coarse-grained simulations provide valuable insights into the effective interactions governing lysozyme solution structure.
  • A balanced approach combining attractive and distributed charge interactions offers a more accurate description of lysozyme solution behavior.