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Updated: May 25, 2026
In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila
Published on: November 27, 2016
A sensitive near-infrared fluorescent probe for caspase-mediated apoptosis: Synthesis and application in cell imaging
Yepeng Luan1, Qiuhong Yang, Yumei Xie
1Department of Medicinal Chemistry, College of Pharmacy, Shandong University, Ji'nan, Shandong, China.
Abstract:
Near-infrared (NIR) absorbing dyes represent an intriguing avenue for extracting biological information from living subjects since they can be monitored with noninvasive optical imaging techniques. We designed and synthesized an imaging agent which contains a NIR fluorochrome (IR780) and peptidyl fluoromethyl ketone (FMK) for caspase-9 imaging of cells undergoing apoptosis. The IR780-FMK fluorescent probe had a Strokes shift of 79 nm and quantum yield 0.75. Prostate cancer DU145 cells undergoing apoptosis were successfully imaged using as little as 0.1 microM of IR780-FMK.
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