Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

New approaches for biocompatibility testing using cell culture.

C J Doillon1, K Cameron

  • 1St-François d'Assise's Hospital, Québec, Canada.

The International Journal of Artificial Organs
|August 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hotspots of Arctic and sub-Arctic marine sediment organic carbon are dominated by the Baltic, Barents and Chukchi Seas.

Communications earth & environment·2026
Same author

Divergent climate impacts despite similar response to temperature in a widespread aerial insectivore.

bioRxiv : the preprint server for biology·2025
Same author

Uterine fibroids and risk of hypertensive disorders of pregnancy - results from a racially diverse high-risk cohort.

medRxiv : the preprint server for health sciences·2024
Same author

Contrasting response of comammox Nitrospira, ammonia oxidising bacteria, and archaea to soil pH and nitrogen inputs.

The Science of the total environment·2024
Same author

Detection of first gammaherpesvirus sequences in Central African bats.

New microbes and new infections·2020
Same author

Pluripotent stem cell derived hepatocytes: using materials to define cellular differentiation and tissue engineering.

Journal of materials chemistry. B·2016

Two modified biocompatibility assays were developed to assess cytotoxic substances from biomaterials. These new methods, focusing on DNA replication, effectively evaluate the safety of medical implants like vascular prostheses.

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Toxicology

Background:

  • Biocompatibility testing is crucial for medical device safety.
  • Existing assays may pose risks of mechanical injury to cells.
  • Assessing cytotoxicity of leached substances from biomaterials requires refined methods.

Purpose of the Study:

  • To introduce two modified biocompatibility assays for enhanced biomaterial evaluation.
  • To analyze the effects of cytotoxic substances leached from polytetrafluoroethylene vascular prostheses.
  • To improve the reliability and safety of cell-based biomaterial testing.

Main Methods:

  • Modified fluid medium assay using graft pieces glued to a screening lid to prevent cell injury.
  • Modified low-temperature grinding technique for "cell inhibition assay" preparation.

Related Experiment Videos

  • Assessment of cell toxicity via the impact on DNA replication.

    • Main Results:

    • The modified assays successfully analyzed the biocompatibility of two polytetrafluoroethylene vascular prostheses.
    • The fluid medium assay modification reduced mechanical damage to cultured cells.
    • The cell inhibition assay modification allowed for effective preparation of biomaterial samples.

    Conclusions:

    • The proposed modified assay methods are suitable for evaluating biomaterial biocompatibility.
    • These refined techniques offer a more reliable approach to assessing cytotoxicity.
    • The study highlights advancements in cell-based testing for medical materials.