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Updated: May 25, 2026

Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture
Published on: August 8, 2013
Measurement of genome-wide RNA synthesis and decay rates with Dynamic Transcriptome Analysis (DTA)
Björn Schwalb1, Daniel Schulz, Mai Sun
1Department of Biochemistry, Ludwig-Maximilians-Universität München, Munich, Germany. schwalb@lmb.uni-muenchen.de
Abstract:
Standard transcriptomics measures total cellular RNA levels. Our understanding of gene regulation would be greatly improved if we could measure RNA synthesis and decay rates on a genome-wide level. To that end, the Dynamic Transcriptome Analysis (DTA) method has been developed. DTA combines metabolic RNA labeling with standard transcriptomics to measure RNA synthesis and decay rates in a precise and non-perturbing manner. Here, we present the open source R/Bioconductor software package DTA. It implements all required bioinformatics steps that allow the accurate absolute quantification and comparison of RNA turnover.
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