Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 25, 2026

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth
02:33

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

Published on: May 17, 2024

Magnetic cryopreservation for dental pulp stem cells.

Sheng-Yang Lee1, Guo-Wei Huang, Jau-Nan Shiung

  • 1School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.

Cells, Tissues, Organs
|January 31, 2012
PubMed
Summary

Magnetic cryopreservation offers a reliable method for preserving dental pulp stem cells (DPSCs). This technique uses less dimethylsulfoxide (DMSO), enhancing its suitability for regenerative medicine applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Santamarine Synergizes With Cisplatin via ROS/JNK Axis to Selectively Induce Apoptosis and DNA Damage in Oral Cancer Cells In Vitro.

Drug development research·2026
Same author

Integrin αvβ3-dependent pathogenic effect and therapeutic effects of DL-N2 combined with EGFR inhibitors in pancreatic adenocarcinoma.

Journal of translational medicine·2026
Same author

Exploring existing methods for calculating dental arch form and curvature.

Journal of dental sciences·2026
Same author

Defect-Rich Gas-Solution Photocatalytic Systems for Nitrogen Reduction Reactions: Enabling Energy and Carbon Reductions.

ACS omega·2026
Same author

Enhancing Surface Hygiene in Healthcare with GNP@PEGDA Composites for Antibacterial and Photothermal Applications.

ACS omega·2025
Same author

Dentin bonding agents and camphorquinone-induced cytotoxicity, 8-isoprostane and prostaglandin production is associated with CYP450, NQO1, NQO2, GST, and GSH peroxidase in human dental pulp cells.

Dental materials : official publication of the Academy of Dental Materials·2025

Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Cell Biology

Background:

  • Tooth banking utilizes magnetic cryopreservation, preserving periodontal ligament cells and dental pulp stem cells (DPSCs).
  • Conventional cryopreservation methods using high dimethylsulfoxide (DMSO) concentrations can be cytotoxic, posing challenges for stem cell therapies.

Purpose of the Study:

  • To evaluate the efficacy of magnetic cryopreservation for human dental pulp stem cells (DPSCs).
  • To compare magnetic cryopreservation with conventional slow-freezing for DPSC viability, function, and DNA stability.

Main Methods:

  • Human dental pulp cells were cryopreserved using magnetic cryopreservation with a serum-free medium (SFM) containing 3% DMSO.
  • Post-thaw assessments included cell viability, adhesion, proliferation, mesenchymal stem cell (MSC) marker expression, differentiation potential (osteogenesis and adipogenesis), and DNA damage.

More Related Videos

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
11:50

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Published on: March 18, 2019

Primary Culture of Dental Pulp Stem Cells
03:45

Primary Culture of Dental Pulp Stem Cells

Published on: May 5, 2023

Related Experiment Videos

Last Updated: May 25, 2026

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth
02:33

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

Published on: May 17, 2024

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
11:50

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Published on: March 18, 2019

Primary Culture of Dental Pulp Stem Cells
03:45

Primary Culture of Dental Pulp Stem Cells

Published on: May 5, 2023

Main Results:

  • An SFM with 3% DMSO was identified as optimal for magnetic cryopreservation of DPSCs.
  • Magnetically cryopreserved DPSCs maintained viability, MSC marker expression, and differentiation capabilities comparable to fresh cells.
  • No significant DNA damage was observed in magnetically cryopreserved DPSCs.

Conclusions:

  • Magnetic cryopreservation is a reliable and effective method for storing DPSCs.
  • The reduced DMSO concentration in SFM for magnetic cryopreservation is advantageous for clinical regenerative medicine applications.