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Updated: May 25, 2026

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization

Published on: April 19, 2015

Nanofiber scaffold gradients for interfacial tissue engineering.

Murugan Ramalingam¹, Marian F Young, Vinoy Thomas

¹Polymers Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.

Journal of Biomaterials Applications

|January 31, 2012

Summary

This summary is machine-generated.

Researchers developed a versatile 2-spinnerette device to create electrospun nanofiber scaffolds with composition gradients. This innovation enhances tissue engineering, particularly for ligaments and tendons, by promoting graded cellular responses.

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Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization

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Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds

Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds

Published on: January 7, 2019

Area of Science:

Biomaterials Science
Tissue Engineering
Nanotechnology

Background:

Developing functional scaffolds for interfacial tissue engineering (ligament, tendon) remains challenging.
Existing methods lack versatility in creating compositional gradients within nanofiber scaffolds.

Purpose of the Study:

To design and demonstrate a novel 2-spinnerette electrospinning device for fabricating nanofiber scaffolds with composition gradients.
To assess the applicability of these gradient scaffolds for interfacial tissue engineering.

Main Methods:

Simultaneous electrospinning of two distinct nanofiber types in an overlapping pattern using a 2-spinnerette device.
Incorporation of amorphous calcium phosphate nanoparticles (nACP) to create composition gradients.
Characterization using dye and assessment of osteoblast (MC3T3-E1) adhesion and proliferation.

Main Results:

Successfully fabricated nanofiber scaffolds with a composition gradient using the 2-spinnerette device.
Demonstrated enhanced osteoblast adhesion and proliferation on regions with higher nACP content, indicating a graded cellular response.
Observed significant release of calcium and phosphate ions from nACP-containing nanofibers, stimulating osteoblast function.

Conclusions:

The 2-spinnerette electrospinning approach offers a versatile method for creating nanofiber gradients from various materials.
These gradient nanofiber scaffolds show significant potential for engineering tissues with specific cellular gradients, such as ligaments and tendons.