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Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
Published on: September 20, 2016
Lipidic phase membrane protein serial femtosecond crystallography
Linda C Johansson1, David Arnlund, Thomas A White
1Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
Nature Methods
|January 31, 2012
Abstract:
X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet.
