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Related Experiment Video

Updated: May 25, 2026

Studying Adipose Endothelial Cell/Adipocyte Cross-Talk in Human Subcutaneous Adipose Tissue
06:35

Studying Adipose Endothelial Cell/Adipocyte Cross-Talk in Human Subcutaneous Adipose Tissue

Published on: April 5, 2024

A novel approach to adipocyte analysis.

Jeffrey H Lee1, John C Kirkham, Michael C McCormack

  • 1Boston, Mass. From Massachusetts General Hospital.

Plastic and Reconstructive Surgery
|January 31, 2012
PubMed
Summary

This study introduces an improved method for analyzing fat cells using automated cell counters, overcoming limitations of manual counting for more accurate viability assessment. This technique enhances research reliability and efficiency in fat grafting studies.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Regenerative Medicine

Background:

  • Traditional adipocyte (fat cell) viability assessment is challenging due to limitations in manual counting methods.
  • Manual cell counting methods like trypan blue are prone to human error, overestimation of viability, and are labor-intensive.
  • Existing methods struggle to accurately analyze adipocyte size and viability, hindering reliable fat grafting research.

Purpose of the Study:

  • To demonstrate an improved, accurate method for adipocyte analysis using automated cell counters.
  • To overcome the limitations of traditional manual cell counting techniques for adipocytes.
  • To enhance the reliability and efficiency of cell viability assessment in fat grafting research.

Main Methods:

  • Human lipoaspirate was processed, and samples were analyzed using both hemocytometers and an automated cell counter with dual viability dyes.

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Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
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Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

Published on: March 17, 2023

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Last Updated: May 25, 2026

Studying Adipose Endothelial Cell/Adipocyte Cross-Talk in Human Subcutaneous Adipose Tissue
06:35

Studying Adipose Endothelial Cell/Adipocyte Cross-Talk in Human Subcutaneous Adipose Tissue

Published on: April 5, 2024

Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
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Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

Published on: March 17, 2023

  • Novel preparation techniques involving carboxymethyl cellulose and formalin were employed to optimize results.
  • Specific cell size gating (40–150 μm) was used to focus on adipocyte populations.

    • Main Results:

    • Automated cell counting significantly reduced variability compared to manual trypan blue counts.
    • Analysis focused on adipocyte-specific sizes (40–150 μm) provided a more precise viable cell count.
    • Carboxymethyl cellulose reduced sampling variability by 80%, and formalin stabilized cell counts over a 4-hour period.

    Conclusions:

    • Automated cell counters offer a more accurate method for identifying viable adipocytes, surpassing traditional counting limitations.
    • The integration of carboxymethyl cellulose and formalin improves the preparation process, decreasing variability and enhancing temporal stability.
    • This novel approach provides an efficient, specific, and reliable method for adipocyte analysis in fat grafting research.