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Functional cloning and characterization of antibiotic resistance genes from the chicken gut microbiome
1Department of Animal Science, The University of Tennessee, Knoxville, Tennessee, USA.
Abstract:
Culture-independent sampling in conjunction with a functional cloning approach identified diverse antibiotic resistance genes for different classes of antibiotics in gut microbiomes from both conventionally raised and free-range chickens. Many of the genes are phylogenetically distant from known resistance genes. Two unique genes that conferred ampicillin and spectinomycin resistance were also functional in Campylobacter, a distant relative of the Escherichia coli host used to generate the genomic libraries.
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