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Updated: May 25, 2026
Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis
Published on: July 23, 2015
Correlating matrix metalloproteinase-9 sinus secretion levels with tissue biopsy levels
Matt A Wilson1, Richard R Orlandi, Matthew A Firpo
1Department of Surgery, Division of Otolaryngology-Head and Neck Surgery, University of Utah, Salt Lake City, UT 84132, USA. matthew.wilson@hsc.utah.edu
Background:
Collecting mucosal biopsies is invasive and creates additional inflammation, hampering a better understanding of nasal and sinus disease evolution and response to treatment. We examine whether sinus secretion collection can replace tissue biopsy for protein determination.
Methods:
Prior to surgical intervention for chronic rhinosinusitis (CRS), a piece of gelatin foam was used to collect secretions from the ethmoid mucosa. A tissue biopsy was then taken from the same location. Matrix metalloproteinase-9 (MMP-9) protein levels were measured in each sample.
Results:
MMP-9 protein levels in secretions and tissues were significantly correlated (p = 0.0033, r = 0.52, by Pearson correlation).
Conclusion:
Secretion collection can replace tissue biopsy for MMP-9 determinations, reducing morbidity. Furthermore, secretion collection allows sequential sampling from the same location.
