Related Experiment Video

Updated: May 25, 2026

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells
10:57

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

Published on: January 12, 2020

[IAPs: a central element in the NF-κB activating signaling pathway]

Jessy Cartier1, Arthur Marivin, Jean Berthelet

  • 1Inserm UMR 866, Faculté de Médecine, Université de Bourgogne, 7, Boulevard Jeanne d'Arc, 21079 Dijon Cedex, France.

Medecine Sciences : M/S
|February 1, 2012
PubMed

Insights

Inhibitor of apoptosis proteins (IAPs) traditionally block cell death. This review reveals their unexpected crucial role in regulating NF-κB signaling pathways, impacting cancer therapy development.

Area of Science:

  • Molecular Biology
  • Cellular Signaling
  • Cancer Research

Context:

  • Inhibitor of Apoptosis Proteins (IAPs) traditionally function by inhibiting apoptosis via caspase binding.
  • Altered IAP expression in tumors makes them key targets for anticancer drug development.
  • Small molecules targeting IAP-caspase interactions are under investigation for therapeutic potential.

Purpose:

  • To review the central role of cellular IAP1 (cIAP1), cellular IAP2 (cIAP2), and X-linked IAP (XIAP) in regulating NF-κB signaling.
  • To explore the unexpected impact of IAP-targeting small molecules on NF-κB activation.
  • To highlight IAPs as crucial regulators beyond apoptosis inhibition.

Summary:

  • IAPs, including cIAP1, cIAP2, and XIAP, are known inhibitors of apoptosis.
  • Small molecules designed to inhibit IAP-caspase interactions unexpectedly modulate NF-κB signaling pathways.
  • This review focuses on the regulatory functions of cIAP1, cIAP2, and XIAP in NF-κB activation.

Impact:

  • Reveals a novel, significant role for IAPs in regulating NF-κB signaling pathways.
  • Suggests that IAP-targeting anticancer therapies may have broader implications for immune and inflammatory responses.
  • Provides a comprehensive overview for researchers investigating IAP function and NF-κB pathways in cancer.

More Related Videos

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α11:27

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Published on: November 2, 2018

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia09:52

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

Published on: December 4, 2018

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-kB-dependent Signaling Pathway02:26

NF-kB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...