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Hypoxic Preconditioning of Marrow-derived Progenitor Cells As a Source for the Generation of Mature Schwann Cells
Published on: June 14, 2017
Schwann-like cell differentiation from rat bone marrow stem cells.
Iraj Ragerdi Kashani1, Zolikha Golipoor, Mohammad Akbari
1Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Iran.
Archives of Medical Science : AMS
|February 1, 2012
Summary
Bone marrow stem cells (BMSCs) successfully differentiated into Schwann-like cells. This process, however, led to significant apoptosis, with 40-50% of cells undergoing programmed cell death.
Area of Science:
- Stem cell biology
- Neuroscience
- Cellular differentiation
Background:
- Bone marrow stem cells (BMSCs) are multipotent cells with potential therapeutic applications.
- Schwann cells are crucial for peripheral nerve function and regeneration.
- Understanding BMSC differentiation into Schwann-like cells is key for regenerative medicine.
Purpose of the Study:
- To differentiate bone marrow stem cells (BMSCs) into Schwann-like cells.
- To quantify the rate of apoptosis during BMSC differentiation.
- To identify specific markers of differentiated Schwann-like cells.
Main Methods:
- BMSCs isolated from rat femur and characterized by cell surface markers.
- Sequential induction using specific pre-inducer and inducer factors.
- Immunocytochemistry, RT-PCR, flow cytometry (Annexin V/propidium iodide), and MTT assay used for analysis.
Main Results:
- Differentiated BMSCs expressed Schwann cell markers (S-100, P75, GFAP) by day 14.
- 40-50% of BMSCs underwent apoptosis during differentiation.
- Remaining cells attached and differentiated successfully.
Conclusions:
- BMSCs can be differentiated into functional Schwann-like cells.
- Apoptosis is a significant factor during this differentiation process.
- This research provides insights into cell survival and differentiation mechanisms.
