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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

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Published on: March 20, 2015

A nanoporous optofluidic microsystem for highly sensitive and repeatable surface enhanced Raman spectroscopy

Soroush H Yazdi¹, Ian M White

¹Fischell Department of Bioengineering, University of Maryland, College Park, Maryland 20742, USA.

Biomicrofluidics

|February 1, 2012

Summary

This study introduces an optofluidic surface-enhanced Raman spectroscopy (SERS) device using a nanoporous microfluidic matrix. The novel design significantly enhances SERS detection performance and offers a simple, portable solution for field applications.

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Area of Science:

Optofluidics
Spectroscopy
Nanotechnology

Background:

Integrating optical biosensors into microfluidic channels often hinders Surface-Enhanced Raman Spectroscopy (SERS) performance.
Optofluidics, combining microfluidics and photonics, has emerged to enhance SERS detection.
Previous optofluidic SERS methods used single nanofluidic channels for analyte preconcentration.

Purpose of the Study:

To develop an improved optofluidic SERS device utilizing a nanoporous microfluidic matrix.
To enhance SERS detection performance and simplify device fabrication and operation.
To demonstrate the device's potential for portable, automated, and point-of-care applications.

Main Methods:

Fabrication of a microfluidic channel containing a 3D nanofluidic network of packed nanoporous silica microspheres.
Utilizing the nanoporous matrix to trap silver nanoclusters and analytes for preconcentration.
Integration of multimode fiber optic cables for simplified optical alignment.

Main Results:

Achieved SERS detection performance improvement of over two orders of magnitude compared to open channels.
Demonstrated a detection limit of 400 attomoles of Rhodamine 6G within a 2-minute loading time.
Successfully detected 12 ppb of malathion in water, showcasing a practical field application.

Conclusions:

The developed optofluidic SERS device with a nanoporous matrix offers superior detection sensitivity and robustness.
The simplified fabrication and integrated optics make the system suitable for portable and automated field detection.
This technology holds promise for point-of-sample and point-of-care diagnostics.