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Updated: May 25, 2026

Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos
Published on: January 30, 2013
In situ hybridization to cellular RNA
R Zeller1, M Rogers, A G Haramis
1University of Utrecht, The Netherlands.
Abstract:
In situ hybridization to cellular RNA is used to determine the cellular localization of specific messages within complex cell populations and tissues. Tissues may either be embedded in paraffin and sectioned on a microtome (see Hybridization Using Paraffin Sections and Cells), or frozen and sectioned in a cryostat (see Hybridization Using Cryosections). RNA contained in the specimens is hybridized to a specific radiolabeled probe (see Synthesis of (35)S-Labled Riboprobes and Synthesis of (35)S-Labled Double-Stranded DNA Probes), which is then detected using film autoradiography or emulsion autoradiography (Chapter 8).
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