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Related Experiment Video

Updated: May 25, 2026

Production and Multi-Parameter Live Cell Fluorescence Lifetime Imaging Microscopy (FLIM) of Multicellular Spheroids
08:43

Production and Multi-Parameter Live Cell Fluorescence Lifetime Imaging Microscopy (FLIM) of Multicellular Spheroids

Published on: August 9, 2024

Cell-based assays using the fluorometric imaging plate reader (FLIPR).

K L Whiteaker1, J P Sullivan, M Gopalakrishnan

  • 1Abbott Laboratories, Abbott Park, Illinois, USA.

Current Protocols in Pharmacology
|February 2, 2012
PubMed
Summary
This summary is machine-generated.

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Identifying novel pharmacophores is key for drug discovery. High-throughput cell-based assays using the Fluorometric Image Plate Reader (FLIPR) enable rapid screening of compounds for receptor/ion channel activity.

Area of Science:

  • Pharmacology
  • Biotechnology
  • Drug Discovery

Background:

  • Identifying novel pharmacophores is crucial for drug discovery and development.
  • High-throughput screening (HTS) of chemical libraries accelerates the identification of potential drug candidates.
  • Cell-based assays are essential for evaluating compound activity on specific biological targets.

Purpose of the Study:

  • To describe the application of the Fluorometric Image Plate Reader (FLIPR) in cell-based kinetic assays.
  • To demonstrate the utility of FLIPR for measuring cellular signaling processes.
  • To highlight FLIPR's role in screening compound libraries for drug discovery.

Main Methods:

  • Utilizing the Fluorometric Image Plate Reader (FLIPR) for simultaneous kinetic measurements.

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  • Performing cell-based assays in 96- or 384-well plate formats.
  • Measuring fluorescence changes indicative of cellular signaling events.

    • Main Results:

    • FLIPR enables rapid, high-throughput screening of cellular signaling processes.
    • The technology facilitates kinetic measurements of fluorescence changes in cell-based assays.
    • Applications include measuring membrane potential changes and intracellular calcium dynamics.

    Conclusions:

    • The FLIPR is a valuable tool for accelerating drug discovery efforts.
    • FLIPR-based assays provide a robust method for evaluating compound activity.
    • This technology supports the identification of novel pharmacophores through efficient screening.