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Updated: May 25, 2026
08:24
Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells
Published on: September 14, 2016
Drug testing in cellular chemotaxis assays.
Mario Mellado1, Carlos Martínez-A, José Miguel Rodríguez-Frade
1Department of Immunology and Oncology, Centro Nacional de Biotecnología/CSIC, Madrid, Spain.
Current Protocols in Pharmacology
|February 2, 2012
Summary
This unit details methods for measuring cell migration. Assays analyze cell movement towards chemotactic gradients and interactions with the endothelial matrix, crucial for understanding tumor cell motility.
Area of Science:
- Cell Biology
- Biophysics
- Cancer Research
Background:
- Cell migration is a fundamental biological process.
- Understanding cell motility is critical in various physiological and pathological contexts, including cancer.
- Existing protocols allow for the study of cell adhesion and migration separately.
Purpose of the Study:
- To describe methods for measuring the cell migration process.
- To provide protocols for analyzing cell adhesion, migration, and invasion.
- To offer an in vitro cell invasion protocol for assessing tumor cell motility.
Main Methods:
- Cell adhesion protocols to study interactions with the endothelial matrix.
- Cellular migration assays for analyzing directed cell movement towards a chemotactic gradient (in vivo and in vitro).
- An in vitro cell invasion protocol.
Main Results:
- Established methods for quantifying cell adhesion to endothelial matrices.
- Validated assays for measuring directed cell migration in response to chemotactic stimuli.
- Developed a comprehensive in vitro protocol to assess combined cell adhesion, migration, and invasion activities.
Conclusions:
- The described methods provide a comprehensive toolkit for studying cell migration.
- These protocols are applicable for both in vivo and in vitro analyses.
- The in vitro cell invasion protocol is particularly useful for evaluating the multifaceted nature of tumor cell motility.
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