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Updated: May 25, 2026

Propagation of Homalodisca coagulata virus-01 via Homalodisca vitripennis Cell Culture
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Propagation of Homalodisca coagulata virus-01 via Homalodisca vitripennis Cell Culture

Published on: September 25, 2014

Identification and remediation of a cell culture virus contamination

Nathan Jones1

  • 1Genzyme Corporation, Cambridge, MA 02142.

PDA Journal of Pharmaceutical Science and Technology
|February 2, 2012
PubMed

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Cell Lines01:16

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...