Related Experiment Video
Updated: May 25, 2026

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles
Published on: July 11, 2025
Molecular identification of three Ompok species using mitochondrial COI gene
Abhishek K Malakar1, Wazir S Lakra, M Goswami
1National Bureau of Fish Genetic Resources, Lucknow 226002, Uttar Pradesh, India.
Abstract:
A DNA-based barcode identification system that is applicable to all animal species will provide a simple, universal tool for the identification of fish species. The barcode system is based on sequence diversity in subunit 1 cytochrome c oxidase (COI) gene. Identification and characterization of fish species based on morphological characters are sometimes found to be erroneous and environmentally affected. There are no studies on the genus Ompok in India at molecular level and species identification of the Ompok is usually carried out through morphological features. A total of 106 samples from three species Ompok pabda, O. pabo and O. bimaculatus were collected from eight sampling sites of seven Indian rivers. One hundred and six sequences were generated from COI region of three Ompok species and 21 haplotypes were observed. The sequence analysis of COI gene revealed three genetically distinct Ompok species and exhibited identical phylogenetic resolution among them. The partial COI gene sequence can be used as a diagnostic molecular marker for identification and resolution of taxonomic ambiguity of Ompok species.
More Related Videos
12:14A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters
Published on: June 13, 2019
07:26Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy
Published on: July 29, 2019
Related Concept Videos
Modern Molecular Taxonomy
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
Animal Mitochondrial Genetics
Applications of Molecular Taxonomy
Evolutionary Relationships through Genome Comparisons
Export of Mitochondrial and Chloroplast Genes