Related Experiment Video
Updated: May 25, 2026
06:12
Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples
Published on: October 25, 2018
Pseudogene recoding revealed from proteomic analysis of salmonella serovars
Ye Feng1, Kun-Yi Chien, Hsiu-Ling Chen
1Genomics Research Center, Harbin Medical University , Harbin, PR China.
Journal of Proteome Research
|February 3, 2012
Summary
Recoding, the reprogramming of mRNA translation, was investigated in Salmonella. Researchers found evidence of recoding mechanisms, suggesting it
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Recoding involves nonstandard mRNA translation rules.
- Host-adapted Salmonella serovars accumulate pseudogenes.
- Pseudogenes are typically non-functional gene remnants.
Purpose of the Study:
- To investigate recoding phenomena in host-adapted Salmonella serovars.
- To identify mechanisms of recoding in bacterial translation.
- To explore the role of pseudogenes in Salmonella proteome.
Main Methods:
- Utilized stable isotope labeling with amino acids in cell culture (SILAC) technology.
- Analyzed the proteome of host-adapted Salmonella serovars.
- Investigated peptide expression from pseudogenes.
Main Results:
- Identified expression of peptides downstream of pseudogene inactivation sites.
- Confirmed the occurrence of recoding in Salmonella.
- Detected both programmed frameshifting and codon redefinition mechanisms.
Conclusions:
- Recoding is a significant phenomenon in bacteria, not limited to pseudogenes.
- Pseudogene recoding plays a role in Salmonella serovars.
- Further research is needed to understand bacterial translation and pseudogene implications.
Related Concept Videos
01:08Evolution of Microbial Genome
Microbial genome evolution is a highly dynamic process shaped by continual gene gain and loss across species and strains. This genomic flexibility allows microorganisms to adapt rapidly to environmental pressures and interactions with other organisms. Central to understanding this diversity is the distinction between the core and pan genomes.The core genome comprises the genes shared by all sampled strains of a species, representing essential functions needed for fundamental cellular processes.
00:46Genomic DNA in Prokaryotes
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic Diversity in Bacteria
Although bacterial genomes are much...
03:21Genome Size and the Evolution of New Genes
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
02:32Exon Recombination
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Exon shuffling follows “splice frame rules.” Each exon has three reading...
02:24Ribosome Profiling
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
02:37Gene Duplication and Divergence
The seminal work of Ohno in 1970 popularized the idea of gene duplication and divergence. DNA sequence comparison studies reveal that a large portion of the genes in bacteria, archaebacteria, and eukaryotes was generated by gene duplication and divergence, indicating its critical role in evolution.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are characterized.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are characterized.