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Updated: May 25, 2026
Semi-automated Imaging of Tissue-specific Fluorescence in Zebrafish Embryos
Published on: May 17, 2014
Highly selective cysteine detection and bioimaging in zebrafish through emission color change of water-soluble
Na Young Kwon1, Daigeun Kim, Geunseok Jang
1Organic and Optoelectronic Materials Laboratory, Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University, Daejeon 305-764, Korea.
Abstract:
A new concept for rapid, label-free cysteine sensing method is proposed via possible naked eye-detection of red-to-blue emission color change. Intermolecular exciton migration in conjugated polyelectrolyte-based assay complex is adopted to enhance selectivity and sensitivity for cysteine sensing by formation and dissociation of polymer-Hg(2+)-thymine assay complex in the absence and presence of cysteine, respectively. The assay complex shows red emission due to cooperative aggregation of conjugated polyelectrolyte, thymine, and Hg(2+). Upon exposure to cysteine, the assay complex dissociates into individual molecules showing transparent, blue-emitting solution, because cysteine extracts Hg(2+) from the assay complex via more favorable binding between cysteine and Hg(2+).
