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Updated: May 25, 2026

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
Published on: October 29, 2019
Oriented imaging of 3D subcellular structures in bacterial cells using optical tweezers
G Carmon1, I Fishov, M Feingold
1Department of Physics, Ben-Gurion University of the Negev, Beer Sheva, Israel.
Abstract:
Using oscillating optical tweezers, we show that controlled alignment of rod-shaped bacterial cells allows imaging fluorescently labeled three-dimensional (3D) subcellular structures from different, optimized viewpoints. To illustrate our method, we analyze the Z ring of E. coli. We obtain that the radial width of the Z ring in unconstricted cells is about 120 nm. This result suggests that the Z ring consists of an extremely sparse network of FtsZ filaments.
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