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Related Experiment Video

Updated: May 25, 2026

Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles

Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles

Published on: April 8, 2012

Packaging in dsRNA viruses.

Leonard Mindich¹

¹Public Health Research Institute Center, UMDNJ - New Jersey Medical School, Newark, NJ 07103, USA. mindicle@umdnj.edu

Advances in Experimental Medicine and Biology

|February 3, 2012

Summary

This summary is machine-generated.

Viruses with segmented genomes precisely package genetic material. Cystoviridae bacteriophages use core particles and motor proteins to package dsRNA segments, ensuring each virus particle receives a complete genome for replication.

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Area of Science:

Virology
Molecular Biology
Structural Biology

Background:

Many viruses feature segmented genomes, requiring precise packaging of each segment into progeny virions.
The Cystoviridae family, encompassing bacteriophages, possesses a three-segmented double-stranded RNA (dsRNA) genome.

Purpose of the Study:

To elucidate the mechanism by which Cystoviridae bacteriophages package their segmented dsRNA genome.
To understand the role of empty core particles and associated protein complexes in genomic segment packaging.

Main Methods:

The study focuses on the structural and functional analysis of Cystoviridae core particles during the packaging process.
Investigated the involvement of hexameric NTPase complexes as motors for transcript translocation.
Examined the function of internal polymerase molecules in synthesizing complementary RNA strands.

Main Results:

Empty dodecahedral core particles are formed and specifically package plus-strand transcripts of the genomic segments.
Packaging involves conformational changes on the particle surface, driven by hexameric NTPase motors.
Internal polymerase enzymes synthesize both plus and minus strand RNA copies.

Conclusions:

Cystoviridae bacteriophages employ a sophisticated packaging mechanism involving conformational changes and motor proteins.
This process ensures the accurate assembly of complete viral genomes within progeny particles.
The findings provide insights into the molecular machinery of viral genome packaging.