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Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS
Published on: May 12, 2011
Protective Effects of Adeno-associated Virus Mediated Brain-derived Neurotrophic Factor Expression on Retinal
Yan Gong1, Zhan-Ping Chang, Ruo-Tong Ren
1Department of Ophthalmology, Chinese PLA General Hospital, No. 28, Fuxing Road, Haidian District, 100853, Beijing, China.
Abstract:
Adeno-associated virus vector plasmid carrying the expression cassette of brain-derived neurotrophic factor (BDNF), pAAV-BDNF, was constructed and packaged into recombinant adeno-associated virus (rAAV-BDNF). The rAAV-BDNF was intravitreally injected into streptzotocin (STZ)-induced diabetic Sprague-Dawley (SD) Rats. Data showed that over-expression of BDNF could increase alive retinal ganglion cell (RGC) number and improve its function in streptzotocin(STZ)-induced diabetic rats, which might be a new method to treat diabetic neuropathy and retinopathy.
