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Allergic Reactions02:06

Allergic Reactions

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Related Experiment Video

Updated: Jul 5, 2026

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)
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Identification and characterization of important cockroach allergens.

R P Stankus1, W E Horner, S B Lehrer

  • 1Department of Medicine, Tulane Medical School, New Orleans, La.

The Journal of Allergy and Clinical Immunology
|November 1, 1990
PubMed
Summary

Cockroach allergens, particularly acidic ones, are key triggers for asthma. This study identified shared acidic allergens between American and German cockroaches, crucial for understanding and managing allergic asthma.

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Area of Science:

  • Immunology
  • Allergology
  • Molecular Biology

Background:

  • Cockroach allergens are major triggers for asthma development and exacerbation.
  • Understanding specific cockroach allergens is vital for effective asthma management.

Purpose of the Study:

  • To identify and characterize key allergens from American (Periplaneta americana) and German (Blattella germanica) cockroach whole body extracts.
  • To investigate the shared allergenic components between these two common cockroach species.

Main Methods:

  • Gel-filtration and chromatofocusing were used to fractionate cockroach extracts.
  • Immunoprinting, skin testing, and Radioallergosorbent Test (RAST) inhibition were employed for allergen characterization.
  • Isoelectric points of allergens were determined using chromatofocusing.

Main Results:

  • Several acidic cockroach allergens with isoelectric points between pH 2.80 and 5.20 were identified.
  • Two major allergens, focusing at pH 3.50 and pH 4.15-4.55, showed high reactivity with sera from cockroach-sensitive individuals.
  • Shared acidic allergens with isoelectric points between pH 3.75 and 4.50 were confirmed between American and German cockroach extracts.

Conclusions:

  • The study identified several acidic cockroach allergens, likely shared between American and German cockroach species.
  • These findings contribute to understanding the molecular basis of cockroach allergy and asthma.
  • The characterized allergens are potential targets for improved diagnostics and therapeutics for cockroach-induced asthma.