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Flow-injection chemiluminescence determination of haemoglobin in the blood

Zoumana Sekou Traore1, Syed Mazhar Shah, Xingguang Su

  • 1Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, People's Republic of China.

Luminescence : the Journal of Biological and Chemical Luminescence
|February 3, 2012
PubMed

In this study, a sensitive and simple flow-injection chemiluminescence (CL) method was developed for the quantitative analysis of haemoglobin. The method is based on the ability of haemoglobin to enhance the CL signal generated by a H(2)O(2) -K(4) Fe(CN)(6) -fluorescein alkaline system enhanced by CdTe quantum dots. Under the optimized conditions, haemoglobin can be detected in concentration range 7.35 × 10(-9) -2.5 × 10(-6) mol/L, with a detection limit (3σ) of 1.8 × 10(-9) mol/L and a relative standard deviation (RSD; for 5 × 10(-7) mol/L haemoglobin) of 2.06% (n = 11). The present CL method was successfully applied for the determination of haemoglobin in three kinds of blood samples taken from an infant, an adult man, an adult woman and two reference samples. Compared with previous reports, the CL method described in this work is simple and rapid, with high sensitivity.

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