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Measuring Phagosome pH by Ratiometric Fluorescence Microscopy
Published on: December 7, 2015
Measurement of pH values in human tissues by two-photon microscopy
Hee Jung Park1, Chang Su Lim, Eun Sun Kim
1Department of Chemistry, Korea University, Anam-ro, Sungbuk-gu, Seoul, Korea.
Angewandte Chemie (International Ed. in English)
|February 3, 2012
Abstract:
pH values go live! A ratiometric two-photon (TP) probe (NP1, see scheme) that has a significant TP action cross-section, high photostability, negligible toxicity, and can estimate pH values in live cells and human tissues by two-photon microscopy is described. NP1 can detect the difference in pH between live cells from the gastroesophageal junction (GEJ) and the lower esophageal sphincter of patients with and without esophagitis.
