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Related Concept Videos

Voltammetric Techniques: Pulse Voltammetry01:17

Voltammetric Techniques: Pulse Voltammetry

Differential-pulse voltammetry (DPV) is a type of voltammetry that involves applying a series of voltage pulses to an electrochemical cell while measuring the resulting current. In DPV, the differential pulse or small potential pulses are superimposed on a linear potential sweep. The magnitude of these pulses is typically small, often in the millivolt range. Each voltage pulse lasts a short duration, usually in the order of a few milliseconds, and is applied at regular intervals along the...
Voltammetric Techniques: Linear-Scan (E vs Time)01:12

Voltammetric Techniques: Linear-Scan (E vs Time)

Polarography is a classical voltammetric technique used to analyze electrochemical reactions. This method applies a linear potential sweep to a dropping mercury electrode (DME), and the resulting current is measured. A dropping mercury electrode is commonly used as the working electrode in polarography. It consists of a capillary tube filled with mercury, where the tiny droplet forms at the tip. This droplet continuously drops from the capillary, creating a new electrode surface for each...
Voltammetry: Overview01:20

Voltammetry: Overview

Voltammetry is an electroanalytical technique in which the current flowing through an electrochemical cell is measured as a function of applied potential, typically under conditions of concentration polarization. The technique provides valuable information about redox-active species, and the current response is plotted as a voltammogram.
A voltammetric cell uses three electrodes: a working electrode, a reference electrode, and an auxiliary electrode. The redox reactions occur in the working...
Voltammetric Techniques: Cyclic Voltammetry01:10

Voltammetric Techniques: Cyclic Voltammetry

Cyclic voltammetry (CV) is an electrochemical technique used to investigate the redox properties of a chemical species. It involves measuring the current response of an electrochemical cell as a function of the applied potential. The setup for cyclic voltammetry typically consists of a working electrode, a reference electrode, and a counter electrode—all immersed in an electrolyte solution. The working electrode is where the redox reaction of interest occurs, while the reference electrode...
Voltammograms: Overview01:16

Voltammograms: Overview

Voltammograms are current plots as a function of applied potential, offering insights into electrochemical systems. The shape of a voltammogram depends on how the current is measured and whether convection (heat transfer by fluid movement) is present or absent.
Shapes of Voltammograms
Voltammetry: Stripping Methods01:13

Voltammetry: Stripping Methods

Anodic Stripping Voltammetry (ASV), Cathodic Stripping Voltammetry (CSV), and Adsorptive Stripping Voltammetry (AdSV) are electrochemical techniques used to determine trace amounts of analytes in solution. These methods involve applying a potential to an electrode and measuring the resulting current.
Anodic Stripping Voltammetry (ASV)
ASV is used to determine metals and metalloids at trace levels. It involves two steps: deposition and stripping. First, a negative potential is applied to the...

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Using Cyclic Voltammetry, UV-Vis-NIR, and EPR Spectroelectrochemistry to Analyze Organic Compounds
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Using Cyclic Voltammetry, UV-Vis-NIR, and EPR Spectroelectrochemistry to Analyze Organic Compounds

Published on: October 18, 2018

Paired pulse voltammetry for differentiating complex analytes.

Dong Pyo Jang1, Inyong Kim, Su-Youne Chang

  • 1Department of Neurologic Surgery, Mayo Clinic, Rochester, MN 55905, USA.

The Analyst
|February 3, 2012
PubMed
Summary
This summary is machine-generated.

Paired-pulse voltammetry (PPV) simplifies complex electrochemical analysis by generating two distinct voltammograms. This novel technique effectively differentiates analytes like dopamine and adenosine, overcoming limitations of fast-scan cyclic voltammetry.

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Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing
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Using Cyclic Voltammetry, UV-Vis-NIR, and EPR Spectroelectrochemistry to Analyze Organic Compounds
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Examination of Rapid Dopamine Dynamics with Fast Scan Cyclic Voltammetry During Intra-oral Tastant Administration in Awake Rats
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Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing
08:19

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

Published on: June 1, 2012

Area of Science:

  • Neuroscience
  • Electrochemistry
  • Analytical Chemistry

Background:

  • Fast-scan cyclic voltammetry (FSCV) is crucial in neuroscience but faces analytical challenges.
  • Microelectrode surface effects and pH changes complicate voltammogram interpretation.
  • Distinguishing between multiple analytes in electrochemical signals remains difficult.

Purpose of the Study:

  • Introduce paired-pulse voltammetry (PPV) to address FSCV limitations.
  • Develop a method to mitigate confounding factors in electrochemical analysis.
  • Enhance the ability to discern and differentiate analytes in complex samples.

Main Methods:

  • Paired-pulse voltammetry (PPV) utilizes a binary waveform with a specific time gap.
  • Two distinct voltammograms (primary and secondary) are simultaneously acquired.
  • The technique was validated using dopamine, adenosine, and pH changes in a flow cell.

Main Results:

  • PPV generated primary and secondary voltammograms with differential analyte responses.
  • Peak oxidation currents decreased by ~50% (dopamine) and ~80% (adenosine) in the secondary scan.
  • pH changes showed a minimal decrease (~4%), allowing for effective discrimination.

Conclusions:

  • PPV significantly reduces the impact of confounding factors like pH changes.
  • The technique differentiates analytes based on their adsorption characteristics at the electrode.
  • PPV offers a robust method for analyzing complex mixtures in electrochemical neuroscience.