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Updated: May 25, 2026

Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution
Published on: October 31, 2014
Facile construction of functional biosurface via SI-ATRP and "click glycosylation"
Wantong Song1, Chunsheng Xiao, Liguo Cui
1Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, PR China.
Abstract:
Construction of high density glycosylated surfaces is important in the investigation of interactions between pathogens and surface carbohydrates. In this work, we provided a flexible method for glycosyl surface fabrication by combination of surface-initiated atom transfer radical polymerization (SI-ATRP) and copper-catalyzed azide-alkyne 1,3-dipolar cycloaddition (CuAAC) reaction. Through this strategy, we got a very high surface glycosyl density of about 4 nmol/cm(2) with the surface "click" efficiency of nearly 50%. Then the carbohydrate decorated surfaces were used to mimic cell surfaces and specific recognition of mannose with Escherichia coli was observed. We believe the methodology provided here can be used as a facile way for construction of a wide range of functional biosurfaces.
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