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Updated: May 25, 2026
A Doxorubicin-Induced Murine Model of Dilated Cardiomyopathy In Vivo
Published on: May 16, 2020
Let-7 g is involved in doxorubicin induced myocardial injury
Jun Fu1, Cike Peng, Wanyi Wang
1Department of Toxicology, Health Science Center, Peking University, No. 38, Xueyuan Road, Haidian District, Beijing 100191, PR China.
Objectives:
To investigate whether let-7 g (miRNA) was involved in doxorubicin-induced cardiotoxicity.
Methods:
Rats were treated with doxorubicin at increasing doses (0mg/kg, 6 mg/kg, 12 mg/kg, 18 mg/kg). Heart rate, pulse pressure and plasma cardiac troponin T concentrations were measured. Primary cultured myocardial cells were incubated with DOX at increasing concentrations (0 μmol/l, 0.004 μmol/l, 0.02 μmol/l, 0.1 μmol/l, 0.5 μmol/l) for 24h. Cellular viability and the beat frequency were measured. For both rats and cultured cells, miRNA content was measured by real-time reverse-transcription PCR.
Results:
All DOX-treated rats had a decrease in heart rate, an increase in pulse pressure compared with control group after injections (p<0.05). Concentration of cTnT was increased significantly in 18 mg/kg group. Content of let-7 g decreased significantly (p<0.05) in 18 mg/kg group in vivo and all the doxorubicin treated group in vitro.
Conclusions:
The down regulation of let-7 g in the myocardial-injury model suggests that let-7 g may play an important role in the development of cardiac disease.
