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Affinity immunoblotting detection of serum monoclonal immunoglobulins reactive with glycosphingolipids
M O Jauberteau1, J M Cook, M Drouet
1Laboratory of Immunology, University Hospital, Limoges, France.
Journal of Immunological Methods
|November 6, 1990
Abstract:
The characterization of serum monoclonal IgM reactive with glycosphingolipid present in the nervous system is of major importance in patients with certain neuropathies. A rapid, sensitive and specific one-step method which permits such a characterization is described. Serum immunoglobulins separated by high resolution agarose electrophoresis are transferred to affinity filters (nitrocellulose sheets coated with glycolipids), and revealed with enzyme-tagged monospecific anti-immunoglobulin antibodies.