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Cell Culture

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

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Related Experiment Video

Updated: May 25, 2026

Live Cell Imaging of Chromosome Segregation During Mitosis

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

Using cultured mammalian cells to study mitosis.

Patricia Wadsworth

Cold Spring Harbor Protocols

|February 4, 2012

Summary

This review covers cell types and methods for studying cell division (mitosis) in live cells. It highlights advancements in understanding chromosome dynamics and cell cycle regulation in cultured mammalian cells.

Area of Science:

Cell Biology
Molecular Biology

Background:

Historically, morphological studies dominated mitosis research.
Recent advancements enable investigation of chromosome dynamics and cell-cycle regulation.

Purpose of the Study:

To review cell types used for live-cell mitosis studies.
To discuss cell lines for mammalian cell mitosis research.
To summarize methods for culturing and studying these cells.

Main Methods:

Live-cell imaging techniques.
Culturing of mammalian cell lines.
Microscopy and molecular analysis.

Main Results:

Overview of diverse cell types employed in mitosis research.

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Last Updated: May 25, 2026

Live Cell Imaging of Chromosome Segregation During Mitosis

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Identification of key cell lines for studying mammalian cell division.

Summary of established protocols for cell culture and analysis.

Conclusions:

The choice of cell type is critical for studying specific aspects of mitosis.
Modern techniques allow detailed investigation of mitosis in cultured mammalian cells.
Standardized methods facilitate reproducible research in cell division.