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Related Concept Videos

Dihybrid Crosses01:18

Dihybrid Crosses

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Trihybrid Crosses02:27

Trihybrid Crosses

Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal chance to...
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Test Cross01:39

Test Cross

Alleles are different forms of the same gene. Humans and other diploid organisms inherit two alleles of every gene, one from each parent.
Test Cross01:39

Test Cross

Alleles are different forms of the same gene. Humans and other diploid organisms inherit two alleles of every gene, one from each parent.

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Embryo Rescue Protocol for Interspecific Hybridization in Squash
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Genetic crosses between Ectocarpus strains.

Susana M Coelho1, Delphine Scornet, Sylvie Rousvoal

  • 1UPMC Université Paris 06, The Marine Plants and Biomolecules Laboratory, UMR 7139, Station Biologique de Roscoff, BP74, 29682 Roscoff Cedex, France. coelho@sb-roscoff.fr

Cold Spring Harbor Protocols
|February 4, 2012
PubMed
Summary
This summary is machine-generated.

This study details a method for Ectocarpus crosses, focusing on distinguishing diploid sporophytes from haploid partheno-sporophytes. This technique enables classical genetic analysis in Ectocarpus.

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Area of Science:

  • Marine Biology
  • Phycology
  • Genetics

Background:

  • Ectocarpus is a model organism for brown algal research.
  • Genetic studies in Ectocarpus are crucial for understanding seaweed biology and evolution.
  • Distinguishing between diploid sporophytes and haploid partheno-sporophytes is a key challenge.

Purpose of the Study:

  • To describe a reliable procedure for conducting genetic crosses in Ectocarpus.
  • To overcome the technical challenge of differentiating sporophyte and partheno-sporophyte generations.
  • To facilitate the application of classical genetic methodologies in Ectocarpus.

Main Methods:

  • Crossing Ectocarpus gametophytes to produce the sporophyte generation.
  • Careful monitoring of progeny to distinguish diploid sporophytes from haploid partheno-sporophytes.
  • Utilizing techniques such as allelic complementation, backcrosses, and outcrosses.

Main Results:

  • A detailed procedure for Ectocarpus crosses is established.
  • Successful differentiation of diploid sporophytes from haploid partheno-sporophytes is achieved through careful monitoring.
  • The method allows for the creation of mapping populations and application of various genetic analyses.

Conclusions:

  • The described crossing procedure is essential for advancing genetic research in Ectocarpus.
  • This methodology opens avenues for detailed genetic analysis, including mutation studies and population mapping.
  • Accurate identification of sporophyte progeny is critical for successful genetic crosses in this species.