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Published on: January 17, 2016
EST-derived genic molecular markers: development and utilization for generating an advanced transcript map of
Shalu Choudhary1, Rashmi Gaur, Shefali Gupta
1National Institute of Plant Genome Research, Aruna Asaf Ali Marg, Post Box No 10531, New Delhi 110067, India.
Summary
This study developed 487 novel expressed sequence tag (EST)-derived markers for chickpea, creating an advanced gene-rich linkage map. This resource enhances molecular breeding in legumes by improving marker density and gene coverage.
Area of Science:
- Plant genetics
- Molecular breeding
- Legume genomics
Background:
- Well-saturated linkage maps are crucial for molecular breeding, particularly in legumes like chickpea.
- Limited availability of simple sequence repeat (SSR) and genic molecular marker (GMM)-based maps hinders chickpea genetic studies.
Purpose of the Study:
- To develop novel EST-derived functional markers for chickpea.
- To construct an advanced, gene-rich linkage map for chickpea using these new markers.
Main Methods:
- Generated 2,496 chickpea ESTs and developed 487 novel EST-derived markers (EST-SSRs, ITPs, ESTPs, SNPs).
- Analyzed parental polymorphism using 872 markers (487 new, 385 published) between C. arietinum and C. reticulatum.
- Integrated genotypic data with existing data to construct an advanced linkage map.
Main Results:
- Developed 487 novel EST-derived markers, including 125 EST-SSRs, 151 ITPs, 109 ESTPs, and 102 SNPs.
- Identified 318 polymorphic markers out of 872 tested between parental lines.
- Constructed an advanced linkage map with 406 loci on eight linkage groups, spanning 1,497.7 cM with an average marker density of 3.68 cM.
Conclusions:
- The study generated a highly advanced, gene-rich chickpea linkage map using solely co-dominant markers.
- This map significantly improves marker density and gene coverage, providing a valuable resource for chickpea molecular breeding and genetic research.
