Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Serum concentrations of antioxidant vitamins and carotenoids are low in individuals with a history of attempted suicide.

Nutritional neuroscience·2007

Same author

Arsenic trioxide induces different gene expression profiles of genes related to growth and apoptosis in glioma cells dependent on the p53 status.

Molecular biology reports·2007

Same author

Role of p38 mitogen-activated protein kinases in cardioprotection of morphine preconditioning.

Chinese medical journal·2007

Same author

Rapamycin inhibits osteoblast proliferation and differentiation in MC3T3-E1 cells and primary mouse bone marrow stromal cells.

Journal of cellular biochemistry·2007

Same author

[Studies on chemical constituents of Salsola collina].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica·2007

Same author

Functional characterization of the Arabidopsis eukaryotic translation initiation factor 5A-2 that plays a crucial role in plant growth and development by regulating cell division, cell growth, and cell death.

Plant physiology·2007

Search research articles

Related Experiment Video

Updated: May 25, 2026

Functional Site-Directed Fluorometry in Native Cells to Study Skeletal Muscle Excitability

Functional Site-Directed Fluorometry in Native Cells to Study Skeletal Muscle Excitability

Published on: June 2, 2023

A selective fluorescent turn-on NIR probe for cysteine.

Xin-Dong Jiang¹, Jian Zhang, Xiangmin Shao

¹The Key Laboratory for Special Functional Materials, Ministry of Education, Henan University, Henan, 475004, China. xdjiang@henu.edu.cn

Organic & Biomolecular Chemistry

|February 4, 2012

Summary

A new fluorescent probe detects cysteine with high selectivity and sensitivity. This near-infrared (NIR) probe utilizes a cleavable 2,4-dinitrobenzenesulfonyl group, enabling a "turn-on" signal for thiol detection.

More Related Videos

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Published on: March 2, 2020

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling

Published on: May 29, 2011

Related Experiment Videos

Last Updated: May 25, 2026

Functional Site-Directed Fluorometry in Native Cells to Study Skeletal Muscle Excitability

Functional Site-Directed Fluorometry in Native Cells to Study Skeletal Muscle Excitability

Published on: June 2, 2023

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Published on: March 2, 2020

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling

Published on: May 29, 2011

Area of Science:

Analytical Chemistry
Biochemistry
Organic Chemistry

Background:

Cysteine, a crucial amino acid, plays vital roles in biological systems.
Accurate detection of cysteine is essential for understanding various physiological and pathological processes.
Development of selective and sensitive probes is critical for biological thiol analysis.

Purpose of the Study:

To develop a novel fluorescent probe for the selective and sensitive detection of cysteine.
To investigate the mechanism of the probe's response to thiols, particularly cysteine.
To explore the application of the probe in biological sample analysis.

Main Methods:

Synthesis of a novel aza-BODIPY based fluorescent dye.
Modification of the dye with a 2,4-dinitrobenzenesulfonyl (DNBS) protecting group.
Spectroscopic characterization (fluorescence emission and quantum yield) of the probe and its response to thiols.

Main Results:

A selective and sensitive 'turn-on' fluorescent near-infrared (NIR) probe for cysteine was successfully developed.
Cleavage of the DNBS group by thiols, such as cysteine, resulted in a significant increase in fluorescence intensity.
The probe exhibited a shift in emission from 734 nm to 755 nm with an enhanced fluorescence quantum yield (Φ(f)) from 0.03 to 0.14.

Conclusions:

The developed probe offers a promising tool for the sensitive and selective detection of cysteine in biological systems.
The 'turn-on' fluorescence mechanism provides a clear signal for thiol detection.
The NIR emission properties are advantageous for biological imaging applications.