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Ligand binding assays in the 21st Century laboratory: automation

Ago B Ahene1, Chris Morrow, David Rusnak

  • 1XOMA Corporation, Berkeley, California 94710, USA. ahene@xoma.com

The AAPS Journal
|February 4, 2012
PubMed
Abstract

No abstract available in PubMed .

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The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
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Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
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