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Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Quantitative Characterization of Liquid Photosensitive Bioink Properties for Continuous Digital Light Processing Based Printing
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Published on: April 14, 2023

Two-photon continuous flow lithography.

Simona C Laza1, Marco Polo, Antonio A R Neves

  • 1National Nanotechnology Laboratory of Istituto Nanoscienze-CNR, Università del Salento, via Arnesano, Lecce, Italy.

Advanced Materials (Deerfield Beach, Fla.)
|February 4, 2012
PubMed
Summary
This summary is machine-generated.

A novel two-photon continuous flow lithography method enables microfluidics-based production of 3D polymeric particles. This technique overcomes traditional limitations, creating sub-micrometer structures with high resolution and smooth surfaces.

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Area of Science:

  • Materials Science
  • Chemical Engineering
  • Nanotechnology

Background:

  • Traditional two-photon lithography has limitations in production speed and scalability.
  • Microfluidics offers precise control for material synthesis.

Purpose of the Study:

  • To report a new microfluidics-based method for polymeric particle production.
  • To overcome limitations of traditional two-photon lithography.

Main Methods:

  • Utilizing two-photon lithography with multiple beams under continuous flow.
  • Implementing microfluidics for controlled particle synthesis.

Main Results:

  • Demonstrated production of polymeric fibers, helical, and bow-tie particles.
  • Achieved sub-micrometer and 3D features with sub-diffraction resolution.
  • Obtained surface roughness as low as 10 nm.

Conclusions:

  • Two-photon continuous flow lithography is a viable technique for advanced polymeric particle fabrication.
  • The method allows for precise control over particle morphology and surface properties.