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Updated: May 25, 2026

An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins
Published on: June 26, 2018
Independent vesicle pools underlie different modes of release during neuronal development
Laura C Andreae1, Naila Ben Fredj, Juan Burrone
1Medical Research Council Centre for Developmental Neurobiology, Kings College London, London SE1 1UL, United Kingdom.
None:
Mature presynaptic terminals release neurotransmitter both in response to activity and spontaneously. We found that axons of rat hippocampal neurons initially show very high levels of exclusively spontaneous release, which progressively switches over to the mature phenotype during synapse formation. These two modes of vesicle cycling derive from distinct pools throughout development and the initiation of activity-dependent release was independent of postsynaptic contacts, suggesting it is an autonomous presynaptic event.
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