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Updated: May 25, 2026

Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery
08:38

Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery

Published on: October 4, 2018

Phospho-specific flow: fixating on the target

Mark Levis1

  • 1Department of Oncology, Johns Hopkins University, Baltimore, MD, USA. levisma@jhmi.edu

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
|February 4, 2012
PubMed

Insights

Confirming targeted therapy effectiveness in oncology is crucial. This study demonstrates phospho-specific flow cytometry for real-time, in vivo target inhibition confirmation.

Area of Science:

  • Oncology
  • Molecular Biology
  • Immunology

Background:

  • Targeted therapies represent a significant advancement in cancer treatment.
  • A key challenge is verifying target engagement within a living organism (in vivo).
  • Current methods may lack the precision or real-time capability to confirm target inhibition.

Purpose of the Study:

  • To present a novel method for confirming in vivo target inhibition.
  • To demonstrate the utility of phospho-specific flow cytometry in oncology research.
  • To provide a real-time assessment of targeted therapy efficacy.

Main Methods:

  • Application of phospho-specific flow cytometry.
  • In vivo experimental models.
  • Analysis of protein phosphorylation states as a marker of target activity.

Main Results:

  • Phospho-specific flow cytometry successfully confirmed target inhibition in vivo.
  • The method provides real-time data on therapeutic response.
  • Demonstrated ability to monitor the effects of targeted therapies.

Conclusions:

  • Phospho-specific flow cytometry is a valuable tool for validating targeted therapies.
  • This technique enables precise, real-time assessment of in vivo target engagement.
  • Facilitates rapid decision-making in oncology drug development and clinical practice.

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