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Updated: May 25, 2026

Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films
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Increased in vitro Cell Proliferation by Chitosan/pGM-CSF Complexes.

E Salva1, S O Turan, J Akbuğa

  • 1Pathology Laboratory, Vocational Health School, Marmara University, Tibbiye Street, Haydarpasa Campuss, 34668, Istanbul, Turkey.

Indian Journal of Pharmaceutical Sciences
|February 4, 2012
PubMed
Summary
This summary is machine-generated.

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Chitosan effectively carries the granulocyte macrophage colony stimulating factor (GM-CSF) gene, enhancing white blood cell production and cell proliferation. This suggests chitosan/pGM-CSF complexes are promising for GM-CSF therapy.

Area of Science:

  • Biotechnology
  • Gene Therapy
  • Materials Science

Background:

  • Granulocyte macrophage colony stimulating factor (GM-CSF) is a hematopoietic cytokine that stimulates white blood cell production.
  • The GM-CSF gene holds potential for treating various pathological conditions.
  • Chitosan is explored as a non-viral gene delivery vector.

Purpose of the Study:

  • To evaluate chitosan's suitability as a carrier for the pORF-hGMCSF plasmid.
  • To assess the impact of chitosan/pGM-CSF complexes on protein production and cell proliferation.
  • To investigate the physicochemical properties and stability of these complexes.

Main Methods:

  • Chitosan/pGM-CSF complexes were prepared at varying N/P ratios.
  • Complex formation, size, and zeta potential were analyzed.
Keywords:
ChitosanMTTcell proliferationpGM-CSF

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  • In vitro transfection efficiency and stability against enzymatic and serum degradation were tested in HeLa cells.

    • Main Results:

    • Full complexation was achieved at chitosan/pGM-CSF ratios of 0.1/1 and higher.
    • Complexes exhibited a size of approximately 132 nm and a zeta potential of +13.7 mV.
    • Chitosan complexes demonstrated protection against enzymatic and serum degradation, and significantly increased gene expression-dependent cell proliferation.

    Conclusions:

    • Chitosan is a suitable carrier for the pORF-hGMCSF plasmid.
    • Chitosan/pGM-CSF complexes enhance gene expression and cell proliferation, showing potential as an alternative to conventional protein treatments for GM-CSF therapy.
    • Further in vivo studies are warranted.