Analytical Method for the Simultaneous Estimation of Hydrochlorothiazide and Metoprolol Tartrate using RP HPLC

The present study deals with the estimation by RP-HPLC of two different drug components hydrochlorothiazide and metoprolol tartrate present in a tablet formulation. It is a simple, fast, precise and accurate high performance liquid chromatographic method. It is performed using phosphate buffer along with methanol as mobile phase, in the proportion of 60:40. The separation is done on a C(18) column and it is estimated at a λ(max) of 226 nm with a flow of 1 ml/min. The detection limits range from a 0.013 to 0.075 mg/ml for hydrochlorothiazide and 0.10 to 0.60 mg/ml for metoprolol tartrate, respectively. The specificity for interference of any peak with main peak of interest is checked. A scan of the individual drug was taken for assuring the λ(max). The system suitability by precision is also checked to ensure the analytical method. The method was found to be accurate and precise for estimation of the two drugs simultaneously.