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Related Experiment Video

Updated: May 25, 2026

Studying Cell Death Initiation Using a Digital Microscope
06:06

Studying Cell Death Initiation Using a Digital Microscope

Published on: November 10, 2023

Early cell death detection with digital holographic microscopy.

Nicolas Pavillon1, Jonas Kühn, Corinne Moratal

  • 1Microvision and Microdiagnostics Group (MVD), STI, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. nicolas.pavillon@a3.epfl.ch

Plos One
|February 4, 2012
PubMed
Summary

Digital holographic microscopy (DHM) offers a label-free method to detect early cell death by measuring cell volume changes. This technique correlates with traditional viability assays, enabling rapid cell death detection.

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Area of Science:

  • Cell Biology
  • Biophysics
  • Neuroscience

Background:

  • Digital holography measures quantitative phase shifts in cells, reflecting cell volume changes.
  • Cell volume regulation and ionic homeostasis are critical in cell death pathways.
  • Digital holographic microscopy (DHM) is applied for label-free, early cell death detection.

Purpose of the Study:

  • To apply DHM for early, label-free detection of cell death.
  • To investigate the correlation between cell volume regulation and cell death.
  • To establish DHM as a method for assessing neuronal cell death.

Main Methods:

  • Utilized DHM to measure phase shifts in mouse cortical neurons.
  • Induced excitotoxicity using L-glutamate to trigger cell volume regulation.

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Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)
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Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing
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Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing

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Last Updated: May 25, 2026

Studying Cell Death Initiation Using a Digital Microscope
06:06

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Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)
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07:38

Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing

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  • Assessed neuronal death using the trypan blue method for viability.

    • Main Results:

    • Quantitative phase signals were obtained from neurons undergoing early volume regulation.
    • DHM-detected cell volume regulation efficiency correlated with subsequent neuronal death.
    • The phase signal determined by DHM indicated cell death occurrence.

    Conclusions:

    • DHM provides a rapid, optical method for early cell death detection.
    • The phase signal is a reliable indicator of cell death.
    • DHM is a valuable tool for studying cell death processes in real-time.